Translational_Unit

Part:BBa_K2705005:Design

Designed by: Danqing Tong   Group: iGEM18_NKU_CHINA   (2018-09-30)


PgltAB-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 671
    Illegal XhoI site found at 572
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was applied to test the repression function of GltC to PgltAB, for which we applied different glutamate level and tested the fluorescence level changes in LL3.


Source

PgltAB was acquired from LL3 by PCR, GFP sequence was from our lab.

References